| Easyfood A/S | 6000 | Kolding |
| Eco2clean ApS | 7800 | Skive |
| Ecolab ApS | 2500 | Valby |
| Egatec A/S | 5250 | Odense SV |
| Ege-Elektronik Specialsensorer ApS | 6200 | Aabenraa |
| Ehrno Flexible A/S | 7400 | Herning |
| Eilersen Electric Digital Systems A/S | 2980 | Kokkedal |
| Elcor Sønderborg N A/S | 6400 | Sønderborg |
| Elektror Airsystems ApS | 3320 | Skævinge |
| Endress+Hauser A/S | 2860 | Søborg |
| Ensinger Danmark | 4100 | Ringsted |
| EPI Denmark A/S | 2500 | Valby |
| Eurofins Steins Laboratorium A/S | 6600 | Vejen |
| Exodraft Energy | 7100 | Vejle |
| Expandit Mobile ApS | 8520 | Lystrup |
| Exposervice ApS | 3460 | Birkerød |
| Erhvervshus Nord | 9900 | Frederikshavn |
| Euro-Vent ApS | 8382 | Hinnerup |